Typical Applications of GC

• Formulation analysis of organic mixtures
• Trace analysis of organic species
• Health and Safety monitoring
• Atmospheric sampling

Typical Applications of HPLC

• Analysis of trace organic materials
• Analysis of ions in liquids
• Raw material testing

Document Title	View
GC & XPS of Thermal processed Ni Cr & Catalytic Activity.

Principle

Chromatographic techniques involve passing a “mobile phase”, containing the sample, through a “stationary phase” which slows the mobile phase to varying degrees dependent on how strongly the components interact. This slowing causes the components to be separated in time.

In GC (Gas Chromatography), the mobile phase is the sample which has been vaporised and carried in an inert gas, typically He, through a column which contains the stationary phase which can either be a solid or liquid coated on a solid support which retards the passage of the mobile phase.

In HPLC (High Pressure/Performance Liquid Chromatography), the mobile phase is the sample dissolved in a solvent which is forced through a column at high pressure, the stationary phase is particles in the column which interact with the mobile phase and retards the passage of different components of the sample to differing degrees.

Features of Laboratory’s GC Capability

GC Instrument - Philips PU 4550 Gas Chromatograph / Megabore/Capillary GC

• Gas Chromatograph fitted with FID and TC detectors
• Temperature range - Ambient to 450 °C
• Temperature programmable - +50 to +350 °C
• Program rate one - 0.01 to 9.99 °C in 0.01 °C /minute steps
• Program rate two - 10 °C to 49.9 °C @ 0.1 °C/ minute steps
• FID Sensitivity - 1 x 10-12 per second for Toluene
• FID Linearity range - 107 decades
• FID Sensitivity - 10-12 Amps
• TC Sensitivity - 20 x 10-9 grams/ml for Toluene
• For formulation analysis, trace analysis, and H&S monitoring of organic species
• A TD4 thermal desorption unit, can be attached for use with diffusive atmospheric sampling apparatus. The diffusive tubes are used for passive sampling of workplace atmospheres, and can be worn on the lapel of operatives where they absorb a representative sample of the workplace atmospheric organics.

HPLC - High Pressure Liquid Chromatography

Features of Laboratory’s HPLC Capability

HPLC Instrument - Philips PU4015/PU4025 Liquid Chromatography System and Varian 5000

• Liquid Chromatography system comprising a Philips PU4015 high-pressure pump and Philips PU4025 UV detector
• Capable of performing conventional, reversed phase or vacancy chromatography
• With a Vydac301IC column, the system can be used for the determination of trace anion contamination
• With the RP18 column the unit can be used for the analysis of organic species, from polymer additives to lacquer formulations to clinical applications
• A programmable gradient elution pump, Varian 5000, allows gradient elution runs for species of widely different polarities

Our site expert on GC / HPLC is Alan Wicks.

Please contact us to discuss how your requirements can be met using this or any of our analytical techniques. We can issue no-obligation quotations once we have discussed your needs and can offer two levels of service “urgent” and “normal”. We also offer preferential rates for longer term analysis contracts. Click here to find out more.