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High performance liquid chromatography, HPLC, is a chromatographic techniques used to separate a mixture of compounds with the aim of identifying, quantifying and purifying the individual components of the mixture. 

Principle of HPLC

In HPLC a sample dissolved in a solvent, known as the mobile phase, is forced through a column at high pressure.  The particles in the column, the stationary phase, interact with the mobile phase and retard the passage of different components of the sample to differing degrees allowing separation of the components in the sample over time dependent on how strongly they interact with the column.

Typical Applications of HPLC

High-performance liquid chromatography (HPLC), often referred to as high-pressure liquid chromatography, is a chromatographic technique that can separate a mixture of compounds used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of the mixture. It can be used for:-

• Analysis of trace organic materials.
• Analysis of ions in liquids.
• Measurement of anions including chloride, iodide, bromide, fluoride, nitrate, nitrite, sulphate / sulphite, etc..
• Finding evidence of exposure to corrosive environments from trace evidence of the presence of sulphates, nitrates, chlorides, fluorides etc..  Physical and chemical evidence can also involve using  SEM/EDX, SIMS and XPS.
• Raw material quality testing and validation.

LPD Lab Services provides a wide range of HPLC analysis and testing solutions such as:

• Method development.
• Method Validation.
• Quality Control Testing.
• Stability Testing.

 Features of Laboratory’s HPLC and Ion Chromatography (IC) Capabilities

The HPLC Instrument is an Agilent HP1050 HPLC system and a separate Liquid Chromatography system comprising a Philips PU4015 high-pressure pump and Philips PU4025 UV detector used for Ion Chromatography (IC).  The laboratory also has a fluorescence detector.

• Capable of performing conventional, reversed phase or vacancy chromatography.
• With a Vydac301IC column, the system can be used for the determination of trace anion contamination.
• With the RP18 column the unit can be used for the analysis of organic species, from polymer additives to lacquer formulations to clinical applications.
• A programmable gradient elution pump allows gradient elution runs for species of widely different polarities.
   

Our site expert on HPLC is Steven Whittle.

		Method Development and Method Validation Analytical method developement and method validation using the laboratory's range of techniques and experience

Please contact us to discuss how your requirements can be met using this or any of our analytical techniques. We can issue no-obligation quotations once we have discussed your needs and can offer two levels of service “urgent” and “normal”. We also offer preferential rates for longer term analysis contracts.